R&D Systems公司ELISpot kits是高灵敏度、基于微孔板,检测细胞分泌细胞因子情况的试剂盒。ELISpot kits包含两种类型的产品:
1. 完整ELISpot Kits
完整ELISpot Kits是一种ready-to-run型的试剂盒,它包含了ELISpot实验所需的一列各种组分:
l 包被好的PVDF底96孔板
l 检测抗体
l Streptavidin-AP
l BCIP/NBT
l 阳性对照
l 稀释液
l 洗涤液
完整ELISpot Kits灵敏度高,可测定单个细胞的细胞分泌状况。使用ELISpot进行高通量检测的时候,仅需要少量的原代细胞。
2. ELISpot Development Modules
ELISpot Development Modules是进行ELISpot实验的另一种选择,由于一些实验需要研究者针对具体情况,比如细胞类型、细胞刺激条件、以及细胞稀释等,优化实验条件。抗体的不同稀释浓度、用何种酶和底物都会影响实验的最终结果。此时完整ELISpot就不是最佳的选择,而ELISpot Development Modules就成了最佳的选择。
Elispot Development Modules仅包含以下两种组分:
l 捕获抗体(Capture Antibody)
l 检测抗体(Detection Antibody)
此外,R&D Systems还有与ELISpot Development Modules配套的产品—ELISpot Blue Color Module,它包含以下组分:
l Streptavidin-AP
l BCIP/NBT
R&D Systems' ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement.
ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.