产品分类

ELIspot试剂盒及研发试剂盒

 

ELISpot kits

 

R&D Systems公司ELISpot kits是高灵敏度、基于微孔板,检测细胞分泌细胞因子情况的试剂盒。ELISpot kits包含两种类型的产品:

1.        完整ELISpot Kits
完整ELISpot Kits是一种ready-to-run型的试剂盒,它包含了ELISpot实验所需的一列各种组分:

l          包被好的PVDF96孔板

l          检测抗体

l          Streptavidin-AP

l          BCIP/NBT

l          阳性对照

l          稀释液

l          洗涤液

完整ELISpot Kits灵敏度高,可测定单个细胞的细胞分泌状况。使用ELISpot进行高通量检测的时候,仅需要少量的原代细胞。

 

2.        ELISpot Development Modules
ELISpot Development Modules
是进行ELISpot实验的另一种选择,由于一些实验需要研究者针对具体情况,比如细胞类型、细胞刺激条件、以及细胞稀释等,优化实验条件。抗体的不同稀释浓度、用何种酶和底物都会影响实验的最终结果。此时完整ELISpot就不是最佳的选择,而ELISpot Development Modules就成了最佳的选择。
Elispot Development Modules
仅包含以下两种组分:

l          捕获抗体(Capture Antibody

l          检测抗体(Detection Antibody

 

此外,R&D Systems还有与ELISpot Development Modules配套的产品—ELISpot Blue Color Module,它包含以下组分:

l            Streptavidin-AP

l            BCIP/NBT

 

Complete ELISpot kits

R&D Systems' ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

ELISpot Development Modules

ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.