Detection of IL-2 and IL-10 Secretion by Mouse Splenocytes. IL-2 (red spots) and IL-10 (blue spots) secretion from mouse splenocytes (50,000 cells/well) stimulated with PMA/Ca2+ ionomycin was detected using the mouse IL-2/IL-10 Dual Color ELISpot Kit (Catalog #ELD5775).

R&D Systems now offers a mouse IL-2/IL-10 Dual Color ELISpot Kit (Catalog # ELD5775). This complete kit is designed to simultaneously detect IL-2 and IL-10-secreting cells at the single cell level. ELISpot assays can be used to monitor immune responses to various treatments and allow antigen-specific CD4⁺ and/or CD8⁺ T cell responses to be easily quantified. These assays are highly reproducible and can be used to measure responses with frequencies well below 1 in 100,000 cells. Additionally, ELISpot assays do not require priorin vitro expansion of cells and are suitable for high-throughput analysis. For more information on our ELISpot Kits, please visit our website at www.RnDSystems.com/go/ELISpot or call 1-800-343-7475.