悬液芯片│MAP
Fluorokine® MultiAnalyte Profiling
(MAP)试剂盒是一种悬液芯片试剂,利用它可以对50µl的血清、血浆或细胞上清样品进行多因子分析。其原理如下图所示:

图中的Beads上连有抗体,Beads本身可以发荧光,不同荧光的Beads带有不同的抗体,用于分析多因子,最终根据Beads的荧光信号和PE的荧光信号确定某种因子的水平。
Fluorokine® MAP需要与Luminex公司的Luminex100、Luminex200,Qiagen公司的LiquidChip或者BioRad公司的Bio-Plex仪器配合使用。
Fluorokine® MAP由基本试剂盒和待测分析物试剂盒构成,两者分别购买。
基本试剂盒(base kit)包括如下组分:
l StandardCocktail
l 微球稀释液
l 生物素抗体稀释液
l Calibrator稀释液
l Streotavidin-PE
l 洗涤液
l Filter-bottom微孔板
l Mixing Bottles
l 板盖
待测分析物试剂盒(analyte kit)包括如下组分:
l 抗体包被的微球
l 生物素标记的检测抗体
Fluorokine® MAP目前有以下产品可选:
l Human Cytokine Panel A (22)
l Human Cytokine Panel B (9)
l Mouse Cytokine Panel (16)
l Rat Cytokine Panel (17)
l Human Obesity Panel (10)
l Human MMP Panel (8)
l Human Adhesion Molecule Panel (4)
Multiplex assays have become highly useful tools for measuring the levels and/or activities of multiple proteins in a single sample. R&D Systems offers both bead-based and antibody-spotted membrane-based assays.
Our bead-based Fluorokine® MultiAnalyte Profiling (MAP) Kits using the Luminex® 200™ platform have the capability of measuring the levels of multiple proteins in a single small sample.
Fluorokine MultiAnalyte Profiling Kits & Reagents
Bead-based kits to measure the levels multiple proteins in a single sample.
Proteome Profiler Arrays
Membrane-based kits to measure multiple proteins in a single sample. R&D Systems Proteome Profiler™ Antibody Arrays offer a convenient way to measure multiple proteins in a single sample using an antibody-spotted membrane assay that requires no specialized equipment.